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ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth

DOI :

10.3791/55879-v

11:19 min

July 3rd, 2017

July 3rd, 2017

7,798 Views

1Institute for Systems Biology, 2Center for Infectious Disease Research

We present a method for quantifying growth phenotypes of individual yeast cells as they grow into colonies on solid media using time-lapse microscopy termed, One-cell Doubling Evaluation of Living Arrays of Yeast (ODELAY). Population heterogeneity of genetically identical cells growing into colonies can be directly observed and quantified.

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