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Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization

DOI :

10.3791/55924-v

July 13th, 2017

July 13th, 2017

7,896 Views

1Department of Entomology, China Agricultural University, 2Bio-tech Research Center, Shandong Academy of Agricultural Sciences

This paper describes a detailed and highly effective RNA in situ hybridization protocol particularly for low-level expressed Odorant Receptor (OR) genes, as well as other genes, in insect antennae using digoxigenin (DIG)-labeled or biotin-labeled probes.

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