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Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

DOI :

10.3791/56728-v

March 30th, 2018

March 30th, 2018

6,281 Views

1Department of Biology, Colby College

An improved protocol is presented for the measurement of transient gene expression from reporter constructs in barley aleurone cells after particle bombardment. The combination of automated grain grinding with 96-well plate enzyme assays provides high throughput for the procedure.

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