Treating Cells with TNFα and Harvesting Cells and Total Protein
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Results: TNFα Regulates the Expression of Rubicon in Trophoblasts from Lean Pregnancies with Female Fetuses
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Conclusion
Transcript
The overall goal of this trophoblast cell culture model is to measure the effect of obesity-related inflammation on the regulation of critical pathways in trophoblasts. The main advantage of this control system is it establish a relationship betwe
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Presented here is a protocol for sampling of human placental villous tissue followed by isolation of cytotrophoblasts for primary cell culture. Treatment of trophoblasts with TNFα recapitulates inflammation in the obese intrauterine environment and facilitates the discovery of molecular targets regulated by inflammation in placentas with maternal obesity.