2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development
3.8K Views
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14:19 min
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March 25th, 2022
DOI :
March 25th, 2022
•Lucile Boutaud*1, Marie Michael*1, Céline Banal2, Damelys Calderon1, Sarah Farcy1, Julie Pernelle1, Nicolas Goudin3, Camille Maillard1, Clémantine Dimartino1, Cécile Deleschaux1, Sébastien Dupichaud4, Corinne Lebreton1, Sophie Saunier1, Tania Attié-Bitach1,5, Nadia Bahi-Buisson1,6, Nathalie Lefort2, Sophie Thomas1
1Imagine Institute, INSERM UMR 1163, Université de Paris, 2Imagine Institute, iPSC Core Facility, INSERM UMR U1163, Université de Paris, 3Necker Bio-image Analysis platform of the SFR Necker, 4Imagine Institute, Cell Imaging Platform, INSERM-US24-CNRS UMS 3633 Structure Fédérative de Recherche Necker, INSERM UMR U1163, Université de Paris, 5Fédération de Génétique, Hôpital Necker-Enfants Malades, Assistance Publique Hôpitaux de Paris, 6Pediatric Neurology, APHP- Necker Enfants Malades Hospital
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Chapters in this video
0:07
Introduction
1:42
Generation of 2D hIPS Cell-Based Models of Neocortical Development
2:56
Generation of Dorsal Forebrain Organoids
4:56
In toto Immunolabeling, Clearing, and Lightsheet Acquisition of Dorsal Forebrain Organoids
7:00
Immunostaining and Clearing of Free-Floating Sections of Dorsal Forebrain Organoids
9:20
Representative Results
13:11
Conclusion
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