JoVE Logo
Faculty Resource Center

Sign In

Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro

DOI :

10.3791/63714-v

5:40 min

May 5th, 2022

May 5th, 2022

2,587 Views

1Australian Institute for Bioengineering and Nanotechnology, The University of Queensland

In this study, we provide a detailed technique for a simple yet robust cortical organoid culture system using standard feeder-free hPSC cultures. This is a rapid, efficient, and reproducible protocol for generating organoids that model aspects of brain senescence in vitro.

Tags

Cortical Brain Organoids

-- Views

Related Videos

article

Author Spotlight: A Unique Mouse Model of Asphyxia-Induced Cardiac Arrest

article

Author Spotlight: Unveiling Neural Coding and Mechanisms of Visual Processing in the Superior Colliculus

article

In Vivo Whole-Brain Imaging of Zebrafish Larvae Using Three-Dimensional Fluorescence Microscopy

article

Author Spotlight: Innovative Techniques and Future Directions in Stroke Research

article

Using the Chick Embryo Brain as a Model for In Vivo and Ex Vivo Analyses of Human Glioblastoma Cell Behavior

article

A Lightweight Drive Implant for Chronic Tetrode Recordings in Juvenile Mice

article

Low-Cost Electroencephalographic Recording System Combined with a Millimeter-Sized Coil to Transcranially Stimulate the Mouse Brain In Vivo

article

Integrated Photoacoustic, Ultrasound, and Angiographic Tomography (PAUSAT) for NonInvasive Whole-Brain Imaging of Ischemic Stroke

article

Author Spotlight: Decoding Mitochondrial Aging

article

Establishing Mixed Neuronal and Glial Cell Cultures from Embryonic Mouse Brains to Study Infection and Innate Immunity

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved