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A Simple and Low-cost Assay for Measuring Ambulation in Mouse Models of Muscular Dystrophy

9.8K Views

05:54 min

December 29th, 2017

December 29th, 2017

9,761 Views

0:05

Title

0:43

Chamber Preparation, Muscle Activation, and Ambulation Recording

1:50

Video Analysis

3:28

Results: Analysis of mdx Ambulation and Representative Effects of Exercise

5:16

Conclusion

Transcript

The overall goal of this assay is to measure voluntary ambulation in mouse models of muscular dystrophy. This method can help answer key questions in the muscular dystrophy field about the progression of muscle weakness in mouse models of muscular

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This protocol describes a flexible, low-cost system for measuring mouse ambulation in an open field activity assay. We show that a 6-minute ambulation assay based on this system detects a decrease in voluntary movement in mdx mice, and accurately distinguishes improvement in a muscle-specific rescue of these animals.

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