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Quantitative Measurement of γ-Secretase-mediated Amyloid Precursor Protein and Notch Cleavage in Cell-based Luciferase Reporter Assay Platforms

DOI :

10.3791/56795-v

January 25th, 2018

January 25th, 2018

6,606 Views

1Laboratory of Molecular Neurobiology, Institute of Cellular and Organismic Biology, Academia Sinica

We have successfully generated two substrate-specific γ-secretase assays. Both cell-based assays presented here are designed to quantify γ-secretase enzymatic activities via the output of firefly luciferase reporters.

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Quantitative Measurement

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