Results: Representative Images of Meiotic Figures with Intact Arm Cohesion and Disrupted Arm Cohesion
11:31
Conclusion
Transcript
The overall goal of this procedure is to generate FISH probes and visualize the state of meiotic arm cohesion in drosophila oocytes during prometaphase one and metaphase one. The main advantage of this technique is that it allows one to assay the
Sign in or start your free trial to access this content
This manuscript presents a detailed method for generating X-chromosome arm probes and performing fluorescence in situ hybridization (FISH) to examine the state of sister chromatid cohesion in prometaphase and metaphase I arrested Drosophila oocytes. This protocol is suitable for determining whether meiotic arm cohesion is intact or disrupted in different genotypes.