JoVE Logo
Faculty Resource Center

Sign In

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

DOI :

10.3791/56932-v

May 6th, 2018

May 6th, 2018

9,885 Views

1Neuro-oncology Research Group, VU Medical Center, 2Exosomes Research Group, Department of Pathology, VU Medical Center

Direct injection of cancer-derived extracellular vesicles (EVs) leads to reprogramming of bone marrow supporting tumor progression; however, which cells mediate this effect is unclear. Herein, we describe a step-by-step protocol to investigate EV-mediated tumor-mesenchymal stem cell (MSC) interactions in vivo, revealing a crucial role for EV-educated MSCs in metastasis.

Tags

Preclinical Mouse Model

-- Views

Related Videos

article

Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies

article

The Colon-26 Carcinoma Tumor-bearing Mouse as a Model for the Study of Cancer Cachexia

article

Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer

article

Determining the Optimal Inhibitory Frequency for Cancerous Cells Using Tumor Treating Fields (TTFields)

article

Analyzing the Communication Between Monocytes and Primary Breast Cancer Cells in an Extracellular Matrix Extract (ECME)-based Three-dimensional System

article

A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model

article

Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells

article

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

article

Capturing Small Molecule Communication Between Tissues and Cells Using Imaging Mass Spectrometry

article

A Mouse Model to Investigate the Role of Cancer-Associated Fibroblasts in Tumor Growth

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved