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LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles

DOI :

10.3791/60705-v

February 1st, 2020

February 1st, 2020

5,525 Views

1Department of Oral and Maxillofacial Diseases, University of Helsinki, 2Helsinki University Hospital

Described here is the use of a methylation-specific probe amplification method to analyze methylation levels of LINE-1 elements in mesenchymal stem cells treated with osteosarcoma-derived extracellular vesicles. Ultracentrifugation, a popular procedure for separating extracellular vesicles from fetal bovine serum, is also demonstrated.

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LINE 1 Methylation Analysis

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