Expressing and Purifying Site-specifically Acetylated Protein
6:21
Biochemically Characterizing Acetylated Protein
9:39
Results
10:37
Summary
필기록
In this work, we will demonstrate a facile protocol to produce homogeneously acetylated proteins at specific sites by using E.coli as the expression host. The reversible lysine acetylation of proteins is one of the most abundant post-translational
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Genetic code expansion serves as a powerful tool to study a wide range of biological processes, including protein acetylation. Here we demonstrate a facile protocol to exploit this technique for generating homogeneously acetylated proteins at specific sites in Escherichia coli cells.