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Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling

DOI :

10.3791/57177-v

March 20th, 2018

March 20th, 2018

13,613 Views

1Department of Biochemistry, Charles University

Evaluating the potency of environmental chemicals and drugs, to be enzymatically bioactivated to intermediates generating covalent DNA adducts, is an important field in the development of cancer and its treatment. Methods are described for compound activation to form DNA adducts, as well as techniques for their detection and quantification.

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Keywords Covalent DNA Adducts

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