Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
8.5K Views
•
12:15 min
•
May 29th, 2019
DOI :
May 29th, 2019
•Tiago Franco de Oliveira*1,2, Antonio Anax Falcão de Oliveira*1, Miriam Lemos3, Mariana Veras3, Paulo Hilário Nascimento Saldiva3,4, Marisa Helena Gennari de Medeiros5, Paolo Di Mascio5, Ana Paula de Melo Loureiro1
1Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, 2Departamento de Farmacociências, Universidade Federal de Ciências da Saúde de Porto Alegre, 3Laboratório de Poluição Atmosfêrica Experimental - LIM05, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, 4Instituto de Estudos Avançados, Universidade de São Paulo, 5Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo
Chapters in this video
0:04
Title
1:20
DNA Extraction
4:18
Quantification by HPLC-UV and Solid Phase Extraction for Analyses
6:20
Quantification by HPLC-ESI-MS/MS
9:19
Quantification of the DNA Lesions
10:36
Results: HPLC-UV and HPLC-ESI-MS/MS Analyses
11:25
Conclusion
Related Videos
14:22
Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development
20.2K Views
08:48
Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System
8.6K Views
11:58
A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells
8.2K Views
11:30
Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage
9.4K Views
05:18
Measuring Carbon Content in Airway Macrophages Exposed to Carbon-Containing Particulate Matters
219 Views
10:37
Automated Measurement of Pulmonary Emphysema and Small Airway Remodeling in Cigarette Smoke-exposed Mice
13.2K Views
08:48
Quantitation of γH2AX Foci in Tissue Samples
14.3K Views
05:13
Quantifying the Level of 8-oxo-dG Using ELISA Assay to Evaluate Oxidative DNA Damage in MCF-7 Cells
795 Views
08:17
Generation of a Chronic Obstructive Pulmonary Disease Model in Mice by Repeated Ozone Exposure
10.8K Views
11:24
Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic In Vivo Exposure to Very Low Doses of Beta- and Gamma-Radiation
11.0K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved