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Manipulation of Ploidy in Caenorhabditis elegans

DOI :

10.3791/57296-v

7:54 min

March 15th, 2018

March 15th, 2018

10,394 Views

1Brooklyn College, Biology Department, City University of New York, 2The Graduate Center, Biology Department, City University of New York, 3Advanced Science Research Center, City University of New York

This method allows for the generation of tetraploid and triploid Caenorhabditis nematodes from any diploid strain. Polyploid strains generated by this method have been used to study chromosome interactions in meiotic prophase, and this method is useful for examining important basic questions in cell, developmental, evolutionary, and cancer biology.

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Ploidy Manipulation

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