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Ploidy Manipulation of Zebrafish Embryos with Heat Shock 2 Treatment

DOI :

10.3791/54492-v

December 16th, 2016

December 16th, 2016

9,739 Views

1Laboratory of Genetics, University of Wisconsin, 2Department of Neurobiology, University of Massachusetts Medical School, 3Interdisciplinary Biomedical Graduate Program, University of Pittsburgh School of Medicine

A modified protocol for ploidy manipulation uses a heat shock to induce a one-cycle stall in cytokinesis in the early embryo. This protocol is demonstrated in the zebrafish but may be applicable to other species.

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