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Ploidy Manipulation of Zebrafish Embryos with Heat Shock 2 Treatment

9.9K Views

11:19 min

December 16th, 2016

DOI :

10.3791/54492-v

December 16th, 2016


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Ploidy Manipulation

Chapters in this video

0:05

Title

0:47

Selecting Females for Egg Collection via Interrupted Mating

1:31

Preparation of Sperm Solution

2:50

UV Treatment of Sperm Solution

3:37

Manual Extrusion of Mature Eggs

4:56

In Vitro Fertilization

5:51

Heat Shock Treatment

7:13

Selection of Embryos with a One-cycle Cytokinesis Stall

8:48

Results: Heat Shock 2 Treatment of Zebrafish Embryos

9:57

Conclusion

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