A Multi-well Format Polyacrylamide-based Assay for Studying the Effect of Extracellular Matrix Stiffness on the Bacterial Infection of Adherent Cells
11.6K Views
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11:51 min
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July 5th, 2018
DOI :
July 5th, 2018
•Chapters in this video
0:05
Title
0:39
Manufacturing Thin Two-layered Polyacrylamide Hydrogels on Multi-well Plates
1:36
Polyacrylamide Hydrogel Fabrication
5:22
Infection of Human Microvascular Endothelial Cells with L. monocytogenes
7:11
Flow Cytometry to Quantify Extracellular-matrix-stiffness Dependent Susceptibility of Host Cells to Infection
8:02
Quantitative Time-lapse Microscopy to Assess Extracellular-matrix-stiffness Dependent L. monocytogenes Dissemination Through Endothelial Cells
9:01
Results: The Effect of Extracellular Matrix Stiffness on L. monocytogenes Infection of HMEC-1 Cells
10:32
Conclusion
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