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Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis

DOI :

10.3791/50809-v

August 9th, 2013

August 9th, 2013

11,453 Views

1Department of Pathology, Sealy Center for Vaccine Development, Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch

This method describes the use of click chemistry to measure changes in host cell transcription after infection with the Rift Valley fever virus (RVFV) strain MP-12. Results can be visualized qualitatively via fluorescence microscopy or obtained quantitatively through flow cytometry. This method is adaptable for use with other viruses.

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