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Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques

10.5K Views

13:13 min

September 25th, 2018

DOI :

10.3791/57776-v

September 25th, 2018


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Keywords Single cell Quantitation

Chapters in this video

0:04

Title

0:46

Gene Expression Assay Preparation and Surface Staining of Viable Cells

3:18

Prepare Cell Collection Plates, Perform FACS Sort, and Generate cDNA

5:17

Multiplex qPCR on the Biomark Platform

11:26

Results: Single-cell Quantitation of mRNA and Surface Protein Expression

12:56

Conclusion

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