Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation

20.8K Views

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09:02 min

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November 26th, 2018

DOI :

10.3791/57787-v

November 26th, 2018

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Andreas Agathangelidis*¹, Lesley Ann Sutton*²^,³, Anastasia Hadzidimitriou¹, Cristina Tresoldi⁴, Anton W. Langerak⁵, Chrysoula Belessi⁶, Frederic Davi⁷, Richard Rosenquist²^,³, Kostas Stamatopoulos¹^,², Paolo Ghia⁸

¹Institute of Applied Biosciences, Centre for Research and Technology Hellas, ²Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, ³Department of Molecular Medicine and Surgery, Karolinska Institutet, ⁴Division of Immunology, Transplantation and Infectious, IRCCS San Raffaele Scientific Institute, ⁵Department of Immunology, Laboratory for Medical Immunology, Erasmus University Medical Center, ⁶Hematology Department, Nikea General Hospital, ⁷Assistance publique - Hôpitaux de Paris (AP-HP), Hopital Pitié-Salpêtrière, Department of Hematology, and UPMC University Paris 06, UMRS 1138, ⁸Division of Experimental Oncology, IRCCS Istituto Scientifico San Raffaele and Università Vita-Salute San Raffaele

Summary

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Keywords Immunoglobulin Gene Sequence Analysis

Chronic Lymphocytic Leukemia

Chapters in this video

0:04

Title

0:57

Density Gradient Separation

3:06

Amplification and Sequencing of IGHV-IGHD-IGHJ Gene Rearrangements

5:26

Sequence Analysis

6:39

Results: Immunoglobulin Gene Sequence Analysis

8:21

Conclusion

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