Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation

DOI :

10.3791/57787-v

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9:02 min

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November 26th, 2018

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19,520 Views

Andreas Agathangelidis^1*, Lesley Ann Sutton^2,3*, Anastasia Hadzidimitriou¹, Cristina Tresoldi⁴, Anton W. Langerak⁵, Chrysoula Belessi⁶, Frederic Davi⁷, Richard Rosenquist^2,3, Kostas Stamatopoulos^1,2, Paolo Ghia⁸

¹Institute of Applied Biosciences, Centre for Research and Technology Hellas, ²Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, ³Department of Molecular Medicine and Surgery, Karolinska Institutet, ⁴Division of Immunology, Transplantation and Infectious, IRCCS San Raffaele Scientific Institute, ⁵Department of Immunology, Laboratory for Medical Immunology, Erasmus University Medical Center, ⁶Hematology Department, Nikea General Hospital, ⁷Assistance publique - Hôpitaux de Paris (AP-HP), Hopital Pitié-Salpêtrière, Department of Hematology, and UPMC University Paris 06, UMRS 1138, ⁸Division of Experimental Oncology, IRCCS Istituto Scientifico San Raffaele and Università Vita-Salute San Raffaele

Chapters

Herein, we present a protocol that details the technical aspects and essential requirements to ensure robust IG gene sequence analysis in patients with chronic lymphocytic leukemia (CLL), based on the accumulated experience of the European Research initiative on CLL (ERIC).

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