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High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain

DOI :

10.3791/59474-v

June 2nd, 2019

June 2nd, 2019

7,638 Views

1BioSystems and Micromechanics Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology, CREATE, 2Department of Material Science and Engineering, Massachusetts Institute of Technology, 3Department of Biological Engineering, Massachusetts Institute of Technology

Using a previously designed device to apply mechanical strain to adherent cells, this paper describes a redesigned substratum geometry and a customized apparatus for high-resolution single-cell imaging of strained cells with a 100x oil immersion objective.

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High resolution Imaging

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