Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1^-/- Mice

7.9K Views

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10:01 min

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October 17th, 2018

DOI :

10.3791/58110-v

October 17th, 2018

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Yongli Zhang*¹^,², Hangjie Zhang*², Wenqiang Ma*³, Kefang Liu¹^,², Min Zhao⁴, Yingze Zhao², Xuancheng Lu⁵, Fuping Zhang⁶, Xiangdong Li³, George F. Gao¹^,²^,⁴^,⁶, William J. Liu¹^,²

¹School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, ²NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, ³State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, ⁴Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science, Chinese Academy of Sciences, ⁵Laboratory Animal Center, Chinese Center for Disease Control and Prevention, ⁶CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences

Summary

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Keywords Zika Virus

T cell Responses

Immunoprivileged Organs

Ifnar1 Mice

Tetramer Staining

Immunopathogenesis

Guillain Barre Syndrome

Testes Injury

Interferon Alpha beta Receptor Knockout

Retro orbital Inoculation

Abdominal Surgery

Single cell Suspension

Red Blood Cell Lysis

Flow Cytometry

Chapters in this video

0:04

Title

1:15

Zika Virus (ZIKV) Infection, Immunization, and Splenic Monocyte Isolation

3:19

Brain and Testicular Monocyte Isolation

6:10

Flow Cytometry

7:26

Results: ZIKV Infection Characterization in Ifnar1^-/- Mice

9:10

Conclusion

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Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1-/- Mice

Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1^-/- Mice