Plate-based Large-scale Cultivation of Caenorhabditis elegans: Sample Preparation for the Study of Metabolic Alterations in Diabetes

6.2K Views

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05:08 min

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August 24th, 2018

DOI :

10.3791/58117-v

August 24th, 2018

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Katharina Kohl¹, Thomas Fleming²^,³, Kübra Acunman¹, Hans-Peter Hammes¹^,⁴, Michael Morcos*¹, Andrea Schlotterer*¹

¹5th Medical Department, Medical Faculty Mannheim, Heidelberg University, ²Department of Internal Medicine, Heidelberg University, ³German Center for Diabetes Research (DZD), ⁴European Center for Angioscience, Medical Faculty Mannheim, Heidelberg University

Summary

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Keywords Caenorhabditis Elegans

Plate based Cultivation

Large scale Cultivation

Nematode Population

Synchronized Population

M9 Buffer

Sample Preparation

Metabolic Alterations

Chapters in this video

0:04

Title

0:27

Large Scale Culture on Plates

1:15

Transfer of Large Amounts of Caenorhabditis elegans

1:52

Harvest of a Large Caenorhabditis elegans Sample

3:20

Protein Isolation for Mass Spectrometry

3:56

Results: Reproducible Quantitative Protein Isolation and Further Applications of Large-scale C. elegans Culture

4:47

Conclusion

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