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In Vivo Hydroxyl Radical Protein Footprinting for the Study of Protein Interactions in Caenorhabditis elegans

DOI :

10.3791/60910-v

8:00 min

April 1st, 2020

April 1st, 2020

6,449 Views

1Department of Pharmaceutical Sciences, University of Maryland

In vivo fast photochemical oxidation of proteins (IV-FPOP) is a hydroxyl radical protein footprinting technique that allows for mapping of protein structure in their native environment. This protocol describes the assembly and set-up of the IV-FPOP microfluidic flow system.

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Keywords In Vivo

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