A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC
12.9K Views
•
11:40 min
•
January 30th, 2018
DOI :
January 30th, 2018
•Chapters in this video
0:04
Title
1:05
Cell Preparation and Irradiation
3:21
Cell Metabolic Activity (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide [MTT]) and Viability Assays, and Trans-Epithelial Electrical Resistance (TEER)
6:12
Western Blot Analysis
9:15
Results: Representative Effects of Caco-2 Cell Radiation Exposure and PBMC Co-culture
11:01
Conclusion
Related Videos
09:52
A Mimic of the Tumor Microenvironment: A Simple Method for Generating Enriched Cell Populations and Investigating Intercellular Communication
10.2K Views
10:55
Analyzing the Communication Between Monocytes and Primary Breast Cancer Cells in an Extracellular Matrix Extract (ECME)-based Three-dimensional System
10.9K Views
07:00
Dual Effects of Melanoma Cell-derived Factors on Bone Marrow Adipocytes Differentiation
6.1K Views
07:44
Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports
7.4K Views
07:44
Monitoring Cancer Cell Invasion and T-Cell Cytotoxicity in 3D Culture
11.3K Views
08:00
Real-Time Detection and Capture of Invasive Cell Subpopulations from Co-Cultures
2.3K Views
12:19
Co-Culture In Vitro Systems to Reproduce the Cancer-Immunity Cycle
744 Views
07:46
Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments
4.5K Views
03:19
Co-culturing BC Cells: Culturing Breast Cancer Cells with Bone Fragments to Study Cancer Cell Proliferation
2.3K Views
05:48
3D Co-culture with Direct Interaction: Co-culturing Cancer Cells with Monocytes to Study Their Interaction
3.3K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved