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Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting

DOI :

10.3791/58974-v

10:57 min

June 6th, 2019

June 6th, 2019

10,651 Views

1Institut für Integrative Neuroanatomie, Charité, Universitätsmedizin Berlin, 2Department for Cytometry and Cellsorting, German Rheumatism Research Center, Leibniz Institute, 3Departments of Genetic and Behavioral Neuroscience, Graduate School of Medicine, Gunma University

This protocol describes a cell sorting based method for the purification and culture of fluorescent GABAergic or glutamatergic neurons from the neocortex and hippocampus of postnatal mice or rats.

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Keywords Primary Cell Culture

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