Subretinal Implantation of RPE on a Carrier in Minipigs: Guidelines for Preoperative Preparations, Surgical Techniques, and Postoperative Care

DOI :

10.3791/63505-v

•

11:06 min

•

November 11th, 2022

•

2,174 Views

¹Eye Clinic, Department of Ophthalmology, University Hospital Giessen and Marburg GmbH, ²Karl Landsteiner Institute for Retinal Research and Imaging, ³Department of Ophthalmology, University Hospital Kralovske Vinohrady and Third Faculty of Medicine, Charles University in Prague, ⁴Institute of Macromolecular Chemistry, Czech Academy of Sciences, ⁵Institute of Animal Physiology and Genetics, Czech Academy of Sciences, ⁶Department of Cell Biology, Faculty of Science, Charles University, ⁷Institute of Experimental Medicine, Czech Academy of Sciences, ⁸Center for Eye Research, Department of Ophthalmology, Oslo University Hospital and Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, ⁹Moorfields Eye Hospitals UAE, ¹⁰Stem Cell Therapies in Neurodegenerative Diseases Lab, Research Center “Principe Felipe”, ¹¹Eye Center Donaustadt, Department of Ophthalmology, Sigmund Freud University

Chapters

The subretinal implantation of retinal pigmented epithelium (RPE) is one of the most promising approaches for the treatment of degenerative retinal diseases. However, the performance of preclinical studies on large-eye animal models remains challenging. This report presents guidelines for the subretinal transplantation of RPE on a cell carrier into minipigs.

Related Videos

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement

Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres

Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring

Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry

TAPE: A Biodegradable Hemostatic Glue Inspired by a Ubiquitous Compound in Plants for Surgical Application

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier

A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat

Real-time Visualization and Analysis of Chondrocyte Injury Due to Mechanical Loading in Fully Intact Murine Cartilage Explants

Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model