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Separation of Spinach Thylakoid Protein Complexes by Native Green Gel Electrophoresis and Band Characterization using Time-Correlated Single Photon Counting

DOI :

10.3791/58470-v

February 14th, 2019

February 14th, 2019

7,895 Views

1Department of Plant Biology, Michigan State University, 2Department of Chemistry, Michigan State University

Here we present a protocol to separate solubilized thylakoid complexes by Native Green Gel electrophoresis. Green gel bands are subsequently characterized by Time Correlated Single Photon Counting (TCSPC) and basic steps for data analysis are provided.

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