JoVE Logo
Faculty Resource Center

Sign In

Lentiviral Vector-mediated Gene Therapy of Hepatocytes Ex Vivo for Autologous Transplantation in Swine Video (Video) | JoVE

DOI :

10.3791/58399-v

November 4th, 2018

November 4th, 2018

7,891 Views

1Department of Surgery, Mayo Clinic, 2Midwest Fetal Care Center, Children's Hospitals and Clinics of Minnesota, 3Department of Surgery, Johns Hopkins, 4Pediatric Surgical Associates

This protocol is intended to describe porcine hepatocyte isolation and ex vivo gene delivery to cure models of metabolic diseases via autologous cell transplantation. Although this particular model enjoys unique advantages that favor successful therapy, the application is a relevant foundation to address additional diseases and indications.

-- Views

Related Videos

article

Ex Vivo Culture of Patient Tissue & Examination of Gene Delivery (Video) | JoVE

article

In Vivo Canine Muscle Function Assay (Video) | JoVE

article

Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice (Video) | JoVE

article

Production and Detection of Reactive Oxygen Species (ROS) in Cancers (Video) | JoVE

article

A Novel Surgical Approach for Intratracheal Administration of Bioactive Agents in a Fetal Mouse Model (Video) | JoVE

article

Neo-Islet Formation in Liver of Diabetic Mice by Helper-dependent Adenoviral Vector-Mediated Gene Transfer (Video) | JoVE

article

Matrix-assisted Autologous Chondrocyte Transplantation for Remodeling and Repair of Chondral Defects in a Rabbit Model (Video) | JoVE

article

Steps for the Autologous Ex vivo Perfused Porcine Liver-kidney Experiment (Video) | JoVE

article

Ex Situ Normothermic Machine Perfusion of Donor Livers Video (Video) | JoVE

article

Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation Video (Video) | JoVE

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved