Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples

1.0K Views

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09:26 min

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June 30th, 2023

DOI :

10.3791/65053-v

June 30th, 2023

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José Gonçalves¹^,²^,³, Priscilla Gomes da Silva⁴^,⁵^,⁶, Tom Koritnik³, Martin Bosilj³, Andrés Torres-Franco¹^,², Israel Diaz¹^,², Elisa Rodriguéz¹^,², Eva Marcos²^,³, João R. Mesquita⁴^,⁵, Pedro García-Encina¹^,²

¹Institute of Sustainable Processes, University of Valladolid, ²Department of Chemical Engineering and Environmental Technology, University of Valladolid, ³Department of Public Health Microbiology, National Laboratory of Health, Environment and Food, ⁴ICBAS - School of Medicine and Biomedical Sciences, Porto University, ⁵Epidemiology Research Unit (EPIUnit), Instituto de Saúde Pública da Universidade do Porto, ⁶Laboratório para a Investigação Integrativa e Translacional em Saúde Populacional (ITR)

Summary

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SARS CoV 2

Wastewater based Epidemiology

Viral RNA Extraction Kit

Centrifugal Ultrafiltration

Outbreak Identification

Diagnostics Protocol

Chapters in this video

0:04

Introduction

1:00

Wastewater and Air Samples Collection and Pre‐Processing

1:55

Quantification of SARS‐CoV‐2 RNA by RT‐qPCR

2:46

Sequencing the Variants

7:37

Results: Detection and Quantification of SARS‐CoV‐2 RNA in Wastewater and Air Samples

8:31

Conclusion

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