November 30th, 2018
•Here, we present a protocol to produce large amounts of recombinant RNA in Escherichia coli by co-expressing a chimeric RNA that contains the RNA of interest in a viroid scaffold and a plant tRNA ligase. The main product is a circular molecule that facilitates purification to homogeneity.
Tags
Related Videos
Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification
Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo
Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli
A Simple Method for High Throughput Chemical Screening in Caenorhabditis Elegans
Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
Use of Alu Element Containing Minigenes to Analyze Circular RNAs
Detergent-assisted Reconstitution of Recombinant Drosophila Atlastin into Liposomes for Lipid-mixing Assays
Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved