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Isolation, Fixation, and Immunofluorescence Imaging of Mouse Adrenal Glands

DOI :

10.3791/58530-v

October 2nd, 2018

October 2nd, 2018

22,366 Views

1Department of Internal Medicine (Division of Metabolism, Endocrinology, and Diabetes), University of Michigan Health System, 2Department of Cell and Developmental Biology, University of Michigan Health System, 3Department of Molecular and Integrative Physiology, University of Michigan Health System, 4Endocrine Oncology Program, University of Michigan Health System, 5Comprehensive Cancer Center, University of Michigan Health System

Here we present a method to isolate adrenal glands from mice, fix the tissues, section them, and perform immunofluorescence staining.

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Isolation

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