Preparation for Patch-Clamp Recording and Confocal Imaging
5:41
Patch-Clamp Recording and Confocal Imaging
9:01
Confocal Imaging Data Analysis
10:21
Results: Whole-Cell Voltage-Clamp Recordings and Visualization of Fusion Events Under Confocal Microscope
11:52
Conclusion
Transcript
Membrane fusion mediates many biological functions. This protocol describes a method to detect fusion pore opening and transmitter release dynamics and distinguish three fusion modes;close fusion, stay fusion, and shrink fusion. The combination of
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This protocol describes a confocal imaging technique to detect three fusion modes in bovine adrenal chromaffin cells. These fusion modes include 1) close-fusion (also called kiss-and-run), involving fusion pore opening and closure, 2) stay-fusion, involving fusion pore opening and maintaining the opened pore, and 3) shrink-fusion, involving fused vesicle shrinkage.