JoVE Logo
Faculty Resource Center

Sign In

Primary Culture of Rat Adrenocortical Cells and Assays of Steroidogenic Functions

DOI :

10.3791/59016-v

March 12th, 2019

March 12th, 2019

7,651 Views

1Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, 2Department of Anatomy, School of Medicine, Kaohsiung Medical University, 3Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University

The hormone secreted by the adrenal cortex is vital for animals against stress and diseases. Here, we present a protocol to culture the primary rat adrenal cells. It could be a good in vitro platform for investigating the mechanisms of the reagent of interest in adrenal steroidogenesis and lipid biosynthesis.

Tags

Keywords Primary Adrenal Cell Culture

-- Views

Related Videos

article

Primary Culture of Adult Rat Heart Myocytes

article

Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.

article

Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells

article

Isolation and Primary Culture of Rat Hepatic Cells

article

Culturing Primary Rat Inner Medullary Collecting Duct Cells

article

Isolation and Culture of Mouse Primary Pancreatic Acinar Cells

article

Viability Assays for Cells in Culture

article

Isolation and Primary Culture of Mouse Aortic Endothelial Cells

article

A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells

article

Isolation and Culture of Primary Aortic Endothelial Cells from Miniature Pigs

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved