CRISPR-Cas9-based Genome Engineering to Generate Jurkat Reporter Models for HIV-1 Infection with Selected Proviral Integration Sites
9.8K Views
•
14:27 min
•
November 14th, 2018
DOI :
November 14th, 2018
•Chapters in this video
0:04
Title
0:42
Choice of Targeted Locus, Guide RNA (gRNA), and Targeting Vector Design
3:08
CRISPR-Cas9-based Targeting of Jurkat Cells
4:36
Generation of Clonal Lines and Screening for Correct Targeting
7:50
Screening of Single-cell Clones by Flow Cytometry and PCR
11:46
Results: Screening and Analysis of Single-cell Clones for Correct Reporter Integration
13:16
Conclusion
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