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JoVE Journal

Developmental Biology

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Analysis of Actomyosin Dynamics at Local Cellular and Tissue Scales Using Time-lapse Movies of Cultured Drosophila Egg Chambers

7.3K Views

10:45 min

June 3rd, 2019

June 3rd, 2019

7,314 Views

0:04

Title

0:57

Cell Segmentation: Generation of a Cell Mask for Time Lapse Movies

2:33

Loading the Generated Cell Mask into Surface Manager

3:36

Analysis of Actomyosin Pulses in Surface Manager at Local Cellular Scale

4:51

Selective Surface Extraction of Curved Epithelial Tissues

8:41

Results: Behavior of Myosin II at the Cellular and Tissue Scale in a Drosophila Egg Chamber

10:13

Conclusion

Transcript

Our protocol helps scientists to analyze subcellular actomyosin network in individual epithelial cells and also allows them to perform similar analysis at multicellular scale in curved epithelial tissues. Our protocol is a nice example of a Fiji a

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This protocol provides a Fiji-based, user-friendly methodology along with straightforward instructions explaining how to reliably analyze actomyosin behavior in individual cells and curved epithelial tissues. No programming skills are required to follow the tutorial; all steps are performed in a semi-interactive manner using the graphical user interface of Fiji and associated plugins.

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