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Probing Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices via Laser Flash Photolysis of Photoactivatable Nicotine

9.2K Views

10:48 min

January 25th, 2019

DOI :

10.3791/58873-v

January 25th, 2019


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Nicotinic Acetylcholine Receptor

Chapters in this video

0:04

Title

0:39

Preparation of Photoactivatable Nicotine (PA-Nic)

3:23

Imaging Neurons with 2-Photon Laser Scanning Microscopy

5:28

Calibration of Uncaging Laser Galvanometers and Pairing of PA-Nic Laser Flash Photolysis with Electrophysiological Recordings

7:47

Results: Representative 2-Photon Laser Scanning Microscopy Imaging and PA-Nic Laser Flash Photolysis Responses

9:47

Conclusion

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