Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan
5.2K Views
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11:39 min
•
September 7th, 2019
DOI :
September 7th, 2019
•1Brain and Spinal Cord Innovative Research Center of Tongji Hospital, School of Life Sciences and Technology, Tongji University and Frontier Science Research Center for Stem Cells of Ministry of Education, 2College of Chemistry and Molecular Engineering, Peking-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, and Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University
Chapters in this video
0:05
Title
1:16
Preparation of Mouse Endothelial Culture in Permeable Support Inserts
1:55
Set-up of Neural-endothelial Co-culture and Ac4ManNAz Labeling System
3:25
Immunofluorescent Staining of Sialoglycoproteins in Expanded Primary NSPCs and Differentiated Neurons
5:25
Purification of Sialoglycoproteins from Expanded Primary NSPCs and Differentiated Neurons
8:45
Results: Identifying Cell Surface Markers by Metabolic Labeling of Sialoglycan
10:47
Conclusion
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