Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy
348 Views
•
08:16 min
•
February 7th, 2025
DOI :
February 7th, 2025
•Chapters in this video
0:00
Introduction
1:04
Metabolic Oligosaccharide Engineering to Tag Sialylated Glycans, CuAAC Labeling of Sialylated Glycans, Immunofluorescence Staining of Cells for Expansion Imaging
3:43
Expansion Microscopy Protocol for Imaging Intracellular Sialylated N-Glycoproteins
5:11
Imaging of Organic Fluorophores Tagged Sialylated N-Glycoproteins
7:26
Representative Results
Related Videos
09:09
A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
8.5K Views
07:27
Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry
5.0K Views
09:25
An Endothelial Planar Cell Model for Imaging Immunological Synapse Dynamics
8.9K Views
Label-free Super-resolution Imaging Enabled by Vibrational Imaging of Swelled Tissue and Analysis
1.6K Views
12:06
Metabolic Glycoengineering of Sialic Acid Using N-acyl-modified Mannosamines
12.6K Views
08:55
Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
9.4K Views
09:37
Imaging the Human Immunological Synapse
14.0K Views
11:39
Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan
5.2K Views
08:23
Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins
23.6K Views
02:38
Reversed-Phase High-Performance Liquid Chromatography: A Robust Method for Quantitation of Fluorescently Labeled and Derivatized Sialic Acids Isolated from Mouse Liver
625 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved