Results: Detection of diGly Peptides Using Nanoflow LC-MS/MS
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The post translational modification of proteins by the small protein ubiquitin is involved in many events in the cell. This study presents an original addition to the toolbox for protein ubiquitination analysis. We use enrichment techniques and ma
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We present a method for the purification, detection, and identification of diGly peptides that originate from ubiquitinated proteins from complex biological samples. The presented method is reproducible, robust, and outperforms published methods with respect to the level of depth of the ubiquitinome analysis.