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Direct Injection of a Lentiviral Vector Highlights Multiple Motor Pathways in the Rat Spinal Cord

DOI :

10.3791/59160-v

March 15th, 2019

March 15th, 2019

8,228 Views

1Shriner's Pediatric Research Center, Temple University

This protocol demonstrates injection of a retrogradely transportable viral vector into rat spinal cord tissue. The vector is taken up at the synapse and transported to the cell body of target neurons. This model is suitable for retrograde tracing of important spinal pathways or targeting cells for gene therapy applications.

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Direct Injection

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