Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy
8.6K Views
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12:15 min
•
April 9th, 2019
DOI :
April 9th, 2019
•Carlos Oscar S. Sorzano*1,2, Laura Martínez-Muñoz*3,4, Graciela Cascio3,5, Eva M. García-Cuesta3, J. Vargas1,6, Mario Mellado3, Jose Miguel Rodriguez Frade3
1Department of Macromolecular Structures, Centro Nacional de Biotecnología, Campus Univ. Autónoma de Madrid, 2Campus Urb. Montepríncipe s/n, Univ. San Pablo CEU, 3Department of Immunology and Oncology, Centro Nacional de Biotecnología, Campus Univ. Autónoma de Madrid, 4Department of Cell Signaling, Centro Andaluz de Biología Molecular y Medicina Regenerativa (CSIC), 5Meyer Cancer Center, 6Department of Anatomy and Cell Biology, McGill Univ.
Chapters in this video
0:04
Title
1:37
Preparation of Biological Samples and Image Acquisition
2:57
Selection of Images and Creation of Masks
5:45
Tracking Particles
8:09
Calculation of the Diffusion Coefficients and Classification of Trajectories
9:10
Calculation of Cluster Size
9:50
Results: Analysis of Dynamic Fluorescence Particle Characteristics
11:24
Conclusion
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