Preparation of Biological Samples and Image Acquisition
2:57
Selection of Images and Creation of Masks
5:45
Tracking Particles
8:09
Calculation of the Diffusion Coefficients and Classification of Trajectories
9:10
Calculation of Cluster Size
9:50
Results: Analysis of Dynamic Fluorescence Particle Characteristics
11:24
Conclusion
Transcription
New microscopy techniques require the development of adequate tools for the correct comprehension of the biological processes that are studied. This protocol describes the image processing steps required for single-molecule tracking, including est
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Here, we present a protocol for single particle tracking image analysis that allows quantitative evaluation of diffusion coefficients, types of motion and cluster sizes of single particles detected by fluorescence microscopy.