June 29th, 2020
•Epigenetic markers are used for white blood cell (WBC) subtyping through the quantification of DNA methylation patterns. This protocol presents a multiplex droplet polymerase chain reaction (mdPCR) method using a thermoplastic elastomer (TPE)-based microfluidic device for droplet generation allowing for precise and multiplex methylation-specific target quantification of WBC differential counts.
Tags
Related Videos
High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
Fluorescence detection methods for microfluidic droplet platforms
High-throughput Protein Expression Generator Using a Microfluidic Platform
Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer
Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR
Magnetic Levitation Coupled with Portable Imaging and Analysis for Disease Diagnostics
Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved