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Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers

8.8K Views

08:03 min

July 15th, 2019

DOI :

10.3791/59868-v

July 15th, 2019


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Salivary Epithelial Cells

Chapters in this video

0:04

Title

0:54

Tissue Digestion and Well Coating with BMM (Basement Membrane Matrix)

2:07

Filtering of Undigested Tissue, RBC Lysis, and Cell Plating

3:53

Subculturing the Salispheres and Maintenance on BMM

5:23

Subculturing the Salispheres on Treated Plastic Tissue Culture Dishes

6:43

Results: Primary Human Salisphere Cells Cultured as Spheres or as a Monolayer

7:10

Conclusion

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