December 31st, 2019
•We describe a method for the characterization of proton-driven membrane transporters in membrane vesicle preparations produced by heterologous expression in E. coli and lysis of cells using a French press.
Tags
Related Videos
Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification
Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes
Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis
A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
Expression, Solubilization, and Purification of Eukaryotic Borate Transporters
Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils
Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved