Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy

0:39

Preparation of Crude Mitochondrion from Maize Developing Kernels

1:45

RNA 5’ Polyphosphatase Treatment and Circularization

2:33

Reverse Transcription and Normalization

3:30

PCR Amplification and Determination of Transcript Termini

4:34

Verification of the cRT-PCR Mapping Results by RNA Gel Blot Hybridization

5:00

Discrimination of Primary and Processed 5’ Ends

5:26

Determination of Maize mRNA Termini Using the Circular RT-PCR-based Strategy

7:05

Conclusion