Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

8.2K Views

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00:07 min

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August 21st, 2019

DOI :

10.3791/60029-v

August 21st, 2019

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Emily A. Brown¹^,², Steven C. Neier³^,⁴, Claudia Neuhauser⁵, Adam G. Schrum⁶^,⁷^,⁸, Stephen E.P. Smith¹^,²^,⁹

¹Center for Integrative Brain Research, Seattle Children's Research Institute, ²Graduate Program in Neuroscience, University of Washington, ³Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Department of Medicine, Harvard Medical School, ⁴Broad Institute of Harvard and MIT, ⁵Department of Mathematics, University of Houston, ⁶Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, ⁷Department of Surgery, School of Medicine, University of Missouri, ⁸Department Bioengineering, College of Engineering, University of Missouri, ⁹Department of Pediatrics, University of Washington

Summary

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Protein Interaction Network

Multiplexed Co Immunoprecipitation

Binary Interactions

Co associations

Signal Transduction

Assay Development

Sample Preparation

Immunoprecipitation

Lysate Normalization

Pipetting Techniques

Antibody Dilution

Chapters in this video

0:04

Title

0:59

Quantitative Multiplex Immunoprecipitation (Day 1)

2:19

Running the Assay (Day 2)

4:40

Data Analysis

6:10

Positive Hits and Visualization

6:57

Results: Representative Protein Interaction Network Dynamic Quantification

7:20

Conclusion

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