JoVE Logo
Faculty Resource Center

Sign In

Establishment and Analysis of Three-Dimensional (3D) Organoids Derived from Patient Prostate Cancer Bone Metastasis Specimens and their Xenografts

DOI :

10.3791/60367-v

February 3rd, 2020

February 3rd, 2020

8,890 Views

1Department of Urology, University of California, San Diego, 2Moores Cancer Center, University of California, San Diego, 3Department of Medicine, University of California, San Diego, 4Department of Radiation Oncology, University of California, Los Angeles, 5Department of Orthopedic Surgery, University of California, San Diego

Three-dimensional cultures of patient BMPC specimens and xenografts of bone metastatic prostate cancer maintain the functional heterogeneity of their original tumors resulting in cysts, spheroids and complex, tumor-like organoids. This manuscript provides an optimization strategy and protocol for 3D culture of heterogeneous patient derived samples and their analysis using IFC.

Tags

3D Organoids

-- Views

Related Videos

article

Labeling of Breast Cancer Patient-derived Xenografts with Traceable Reporters for Tumor Growth and Metastasis Studies

article

Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma

article

Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts

article

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

article

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines

article

Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts

article

Enhanced Viability for Ex vivo 3D Hydrogel Cultures of Patient-Derived Xenografts in a Perfused Microfluidic Platform

article

Drug Screening of Primary Patient Derived Tumor Xenografts in Zebrafish

article

The Establishment and Utilization of Patient Derived Xenograft Models of Central Nervous System Metastasis

article

High-Throughput In Vitro Assay using Patient-Derived Tumor Organoids

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved